Pluto Bioinformatics

GSE98679: Hippocampal single-nucleus sequencing identifies a unique transcriptional signature that is predictive of reactivity in dentate granule neurons

Bulk RNA sequencing

Neuronal circuits are constantly remodeled in response to activity, but the extent and the specificity of activity-induced transcription are unknown. In this study, we used single nucleus RNA-seq to compare transcriptional responses to activity throughout the hippocampus following exposure to a novel environment. By comparing activated and non-activated nuclei, we found that dentate granule cells (DGCs) have a unique transcriptional response to activity that differs in both magnitude and quality compared to CA1 pyramidal cells and vasoactive intestinal polypeptide (VIP) interneurons. We further used single nuclei RNA-seq to compare transcriptional responses to activity throughout the dentate gyrus following exposure to two contexts and identified that DGCs generate a transcriptional signature that is predictive of reactivity. SOURCE: Fred,H,Gage (gage@salk.edu) - The Salk Institute for Biological Studies