Pluto Bioinformatics

GSE95448: Single cell RNA-seq of 312 basal epithelial cells from the mammary glands of pregnant and adult mice

Bulk RNA sequencing

Mammary glands were collected from 8 pregnant (12.5 day) mice and 8 adult (10 week old) female mice. Basal epithelial cells were FACS sorted. Freshly sorted cells were submitted to a Fluidigm C1 System machine for single cell capture and cDNA synthesis. Cells were visualized under the microscope to ensure integrity of the captured single cells prior to cDNA preparation. Libraries were prepared using the Nextera XT kit and sequencing was carried out on an Illumina NextSeq 500 to achieve paired-end 75 bp reads. SOURCE: Gordon,K,Smyth (smyth@wehi.edu.au) - Smyth Walter and Eliza Hall Institute of Medical Research