Pluto Bioinformatics

GSE88850: Assessing characteristics of RNA amplification methods for single cell RNA sequencing

Bulk RNA sequencing

We conducted a large-scale control experiment to assess the transfer function of three scRNA-seq methods and factors modulating the function. Our approach was to dilute bulk total RNA (from a single source) to levels bracketing single-cell levels of total RNA (10 pg and 100 pg) in replicates and amplifying the RNA to levels sufficient for RNA sequencing. SOURCE: Junhyong KimJunhyong Kim University of Pennsylvania