Members of the SMYD family of Histone Lysine Methyltransferases, including SMYD3, were shown to be involved in both cardiac and skeletal myogenesis. Currently, very little is known about their mechanisms of action and their potential target genes. To study the cellular and molecular function of SMYD3 in skeletal muscle differentiation, we used loss and gain of function approaches in C2C12 myoblasts. We used RNA sequencing (RNA-seq) to generate gene expression profiling during myogenic differentiation under SMYD3 transient knockdown by siRNAs or SMYD3 stable overexpression in C2C12. We identified multiple genes whose expression is significantly affected by Smyd3 levels, among them the key myogenic regulatory factor (MRF) Myogenin. SOURCE: ROBERTA CODATO (roberta.codato@univ-paris-diderot.fr, roberta.codato@gmail.com) -  Paris Diderot University - UMR 7216 Epigenetics and Cell Fate

Pluto Bioinformatics

GSE137954: Gene Expression Profiling of siRNA Knockdown and overexpression of SMYD3 in C2C12