Purpose:The goal of this study are to understand the role of miceoglia and astrocyte in both physiological and pathological conditions. Methods: Microglia and astrocyte were isolated from 5 time points WT/APP-PS1 mice in triplicate. mRNA were generated by SMART seq2 using Illumina Novaseq platform. Conclusions:  Using DESeq2 to sequence polyA-selected mRNAs from microglia and astrocytes isolated from whole brain. We mapped >85% of reads for all of our sample. We found good reproducibility between the replicates SOURCE: jie pan (panj@bjmu.edu.cn) -  Shenzhen	Peking	University	– The	Hong	Kong	University	of	Science	and	Technology	Medical	Center

Pluto Bioinformatics

GSE137028: Transcriptomic profiling of microglia and astrocytes throughout aging