Pluto Bioinformatics

GSE80960: Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis (RNA-Seq)

Bulk RNA sequencing

We depicted a genome-wide integrative view of DNA methylome by reduced representation bisulfite sequencing (RRBS) and transcriptome by RNA-seq during brown and white adipocyte differentiation. Our analysis demonstrated that DNA methylation is a stable epigenetic signature for brown and white cell lineage before, during and after differentiation. When comparing white to brown adipocytes at all three time points of differentiation, we identified five members of the Hox family whose expression levels were anti-correlated with promoter methylation, suggesting a regulatory role of DNA methylation in transcription. SOURCE: Lei Sun DUKE NUS