Repurpusing cationic amphiphilic drugs (CADs) may be enhance the effect of chemotherapy in cancer treatment. In order to understand the molecular mechanism, we created cell lines resistant to the CAD siramesine by growing them for 6 months in increasing concentrations of siramesine. Parental and resistant cell lines were subjected to RNAseq and revealed diffentially expressed genes in ca+ and cAMP signalling pathways. SOURCE: Mikkel Rohde (mr@cancer.dk) - Cell Death and Metabolism Danish Cancer Society

Pluto Bioinformatics

GSE130363: Transcriptional changes in the breast cancer cell line MCF7 rendered resistant to the cationic drug siramesine