Pluto Bioinformatics

GSE155517: Temporal study of the effect of acute systemic inflammation on the cerebral endothelial cells (CECs) and microglia in mice

Bulk RNA sequencing

To capture the global gene expression patterns in the CECs and microglia following acute systemic inflammation, we injected the mice with LPS and sacrificed them after 30 min, 1 hr and 2 hrs, immediately followed by the isolation of the brains and dissociation to yield single cell suspension, immunolabelling and finally flow assisted cell sorting of CECs (CD45- CD13- CD31+) and microglia (CD45 low-mid CD11b+). RNA was then isolated from the cells followed by RNA-seq. SOURCE: Mahesh Chandra KodaliFrancesca-Fang Liao Lab University of Tennessee Health Science Center