Pluto Bioinformatics

GSE93416: Expression Profiling of MEFs and SKOM induced reprogramming cells after modulation of Cell cycle and Dnmt1 expression

Bulk RNA sequencing

To generate association of DNA methylation level and gene expression profile, we applied RNA-sequencing on mouse embryonic fibroblasts (MEFs) after the modulation of cell cycle and Dnmt1 expression level. To characterize how these factors affect somatic reprogramming, samples of MEFs induced by Yamanaka factors (Sox2, Klf4, Oct4, cMyc) was also included. The cell cycle was accelerated by shRNA targeted to p53, and the expression of Dnmt1 was manipulated by either over expression or knock down. SOURCE: Hui Zheng (zheng_hui@gibh.ac.cn) - Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences