Modulation of metabolism by 5% (physiological) oxygen during reprogramming has been shown to improve reprogramming efficiency, yet it is not known how it may affect the transcriptome and physiology of iPSC. RNA-seq analysis highlighted that iPSC derived under 20% oxygen displayed transcriptomic instability. Consequently, inappropriate modulation of metabolism by atmospheric oxygen during reprogramming significantly impacts the resultant iPSC transcriptional landscape. SOURCE: James Spyrou (jspyrou@student.unimelb.edu.au) -  The University of Melbourne

Pluto Bioinformatics

GSE126785: Transcriptomic analysis of iPSC and ESC challenged with atmospheric or physiological oxygen