Pluto Bioinformatics

GSE100881: Analysis of RNA-seq libraries for conditional knock-out of transcription factors in adult mouse pancreas

Bulk RNA sequencing

The purpose of this study is to determine the roles and hierarchy of the transcription factors Foxa2, Gata4, Nr5a2, and Ptf1a in the control of the physiology of the adult exocrine pancreas. Knockouts of each gene were induced by tamoxifen treatment of mice bearing a Ptf1aCreER allele as well as floxed alleles of the gene(s) to be knocked out. Four to eight RNA-seq libraries were analyzed for control animals, knockouts of each single gene, pairwise knockouts of Ptf1a & Foxa2, Ptf1a & Gata4, Ptf1a & Nr5a2, Foxa2 & Gata4, Gata4 & Nr5a2, and a four gene knockout of Ptf1a, Foxa2, Gata4, and Nr5a2 (eleven different genotypes in all). SOURCE: Galvin Swift (galvin.swift@utsouthwestern.edu) - Ray MacDonald U TX Southwestern Medical Center