Pluto Bioinformatics

GSE74596: Innate-like functions of natural killer T cell subsets result from highly divergent gene programs [single_cell_RNA-seq]

Bulk RNA sequencing

Natural killer T (NKT) cells have immune stimulatory or inhibitory effects on the immune response that are context-dependent. This may be attributed in part to the existence of functional NKT cell subsets; however, these functional subsets have only been characterized on the basis of differential expression of a few transcription factors and cell surface molecules. Here we have analyzed purified populations of thymic NKT cell subsets at both the transcriptomic and epigenomic levels, and by single-cell RNA sequencing. Our data indicate that despite their similar antigen specificity, the functional NKT cell subsets are highly divergent populations characterized by many gene expression and epigenetic differences. Therefore the thymus imprints innate-like NKT cells with novel combinations of properties, including differences in proliferative capacity, homing, and effector functions that were not previously anticipated. SOURCE: Pandurangan Vijayanand (vijay@lji.org) - La Jolla Institute for Allergy and Immunology