Pluto Bioinformatics

GSE153303: The response of A549 transcriptome to RNA Pol II slowdown with alpha-amanitin and DRB

Bulk RNA sequencing

According to current estimates, more than 40% of nascent introns are spliced co-transcriptionally and, consequently, the speed of RNA polymerase II elongation must fundamentally affect the rate of RNA folding, spliceosome assembly, and the resulting mature transcript. RNA-Seq data was generated using RNAPII elongation inhibitors DRB and a-amanitin in A549 cells and used to look at genome-wide splicing changes SOURCE: Dmitri,D,Pervouchine (pervouchine@gmail.com) - Skolkovo Institute of Science and Technology