Pluto Bioinformatics

GSE149156: Transcriptome and Retrotranscriptome profiling in HIV infected women with Breast Cancer (RNA-seq)

Bulk RNA sequencing

In order to determine the impact of HIV infection on the breast cancer microenvironment, we sequenced total RNA from formalin-fixed paraffin-embedded (FFPE) breast cancer samples of 10 women (4 HIV-positive: 6 HIV-negative) for studying the transcriptome and retrotranscriptome of those. We performed RNA extraction from samples stored for more than 5 years, cDNA library preparation, total RNA sequencing (RNA-seq) and differentially expressed genes between conditions were analyzed. SOURCE: Matthew Bendall Weill Cornell Medicine