Pluto Bioinformatics

GSE85197: RNA-seq of N2a cells treated with 100 nM Dexamethasone for 4 hours +/- siARGLU1

Bulk RNA sequencing

ARGLU1 was identified in a screen for new modulators of glucocorticoid signaling in the CNS. RNA-seq of neuronal cells siARGLU1 revealed significant changes in the expression and alternative splicing of distinct genes involved in neurogenesis. Treatment with dexamethasone, a GR activator, also induced changes in the pattern of alternatively spliced genes, highlighting an underappreciated global mechanism of glucocorticoid action in neuronal cells. Thus, in addition to its basal role, ARGLU1 links glucocorticoid-mediated transcription and alternative splicing in neural cells, providing new avenues from which to investigate the molecular underpinnings of cognitive stress disorders. SOURCE: Carolyn,L,Cummins (carolyn.cummins@utoronto.ca) - University of Toronto