Pluto Bioinformatics

GSE151356: RNAseq anaysis of entorhinal cortex layer II neurons upon Ptbp1 modulation

Bulk RNA sequencing

Purpose: To identify gene expression changes in entorhinal cortex layer II (ECII) neurons upon Ptbp1 modulation (silencing and overexpression) Method: bacterial artificial chromosome - Translating Ribosome Affinity Purification (bacTRAP) to isolate actively translated mRNA in ECII neurons, 2 weeks after stereotaxic injection of an AAV1 vector in the EC of ECII-bacTRAP mice; followed by RNAseq. Note: Ptbp1 was significantly overexpressed in the overexpression experiment, but no silencing was achieved with the silencing vector, probably because of tight control of Ptbp1 expression SOURCE: Jean-Pierre Roussarie (jroussarie@rockefeller.edu) - Laboratory of Molecular and Cellular Neuroscience The Rockefeller University