Pluto Bioinformatics

GSE95280: The hypoxic landscape of melanoma

Bulk RNA sequencing

Micro-environment played an important role in the disease progression and overall survival. More recently, hypoxic signature were identified as one of the key signatures of innate anti-PD1 resistance, however, relatively little had been done to identify the key molecular signature of hypoxic response in melanoma and how these may correlated with other know signatures which correlate with poor prognosis. In this study we performed large scale study which integrate ChIP-seq of HIF (in one cell line) and RNA-seq (in three commonly used melanoma cell lines) to identify a common melanoma hypoxic signature. The finding were used to further integrated with our other genome wide which focus on MITF (the master regulator of the melanocyte lineage and a melanoma lineage oncogene, GSE77609) to elucidate the intricate interplay between MITF and HIF and how this interplay define a melanoma specific response to hypoxia. SOURCE: Pakavarin Louphrasitthiphol (pakavarin.louphrasitthiphol@ludwig.ox.ac.uk) - Prof. CRGoding Ludwig Institute for Cancer Research