To investigate the role of CYP2B in lipid metabolism, a Cyp2b triple knockout mouse lacking Cyp2b9, Cyp2b10, and Cyp2b13 was developed using CRISPER/Cas9. Wildtype (WT) and Cyp2b-null mice were fed a normal diet (ND) or a 60% high-fat diet (HFD) for 10 weeks. RNA was extracted from the livers of male mice from all treatment groups and used for RNA seqencing. RNAseq data demonstrated that hepatic gene expression in ND-fed Cyp2b-null mice is similar to HFD-fed WT mice, indicating that Cyp2b-null mice are reacting as if they are receiving a HFD even if they are not. Gene ontology and KEGG pathways show perturbations in lipid metabolism pathways, including PUFA metabolism, fatty acid elongation, and glycerophospholipid metabolism. SOURCE: William,S,Baldwin (baldwin@clemson.edu) - Dr. William Baldwin Clemson University

Pluto Bioinformatics

GSE120761: Cyp2b-null male mice are susceptible to high-fat diet-induced obesity due to changes in responses to hepatic lipids as measured by RNAseq