Pluto Bioinformatics

GSE100473: Effect of O-GlcNAc transferase deficiency on intestinal epithelial cells in mice

Bulk RNA sequencing

O-GlcNAcylation is the modification of serine and threonine residues with beta-N-acetylglucosamine (O-GlcNAc) on intracellular proteins. To investigate the role of protein O-GlcNAcylation on intestinal homeostasis, we generated intestinal epithelial cell (IEC)-specific O-GlcNAc transferase (OGT) knockout in mice. The KO mice developed spontanous intestinal inflammation. To determine the underlying molecular mechanisms, we performed RNA sequencing of ileum and colon epithelial cells of wildtype and IEC-OGT KO mice. SOURCE: Hai-Bin Ruan (hruan@umn.edu) - University of Minnesota