Pluto Bioinformatics

GSE100469: 4sU-seq of HFF exposed to salt and heat stress

Bulk RNA sequencing

Primary human foreskin fibroblasts (HFF) were exposed to either salt stress (80mM KCl) or heat stress (44C). Newly transcribed RNA was labelled by adding 500M 4-thiouridine (4sU) to the cell culture media for 1h. Total cellular RNA was isolated using Trizol. Newly transcribed RNA was purified following the protocol described in Raedle et al. JoVE 2013. SOURCE: Caroline Friedel Ludwig-Maximilians-Universität München