Purpose:  Identification of osteoclast derived coupling factors using Denosumab as a biological probe;	Method: harvested RNA from centrifuged bone biopsies (heterogeneous population containing bone lining cells, osteoblasts, osteoclasts and osteocytes, without in vitro culture;	Results:  Identification of genes differentially regulated in denosumab treated participants that correlate with bone remodeling indices;	Conclusions:  Using this approach we identified denosumab regulated genes that correlate with bone remodeling in the untreated participants and may represent novel osteoclast derived coupling factors. Of interest, we identified DPP4 to be suppressed in denosumab treated participants, providing a novel link between bone remodeling and energy metabolism. SOURCE: Sundeep Khosla Mayo Clinic

Pluto Bioinformatics

GSE141595: Identification of osteoclast coupling factors in humans reveals links between bone remodeling and energy metabolism I