Pluto Bioinformatics

GSE120386: RNA-seq, ChIP-seq and single cell RNA-seq of human skin Langerhans cells

Bulk RNA sequencing

Langerhans cells (LCs) in the epidermis promote immune homeostasis, efficiently activating tolerogenic and immunogenic T cell responses. To understand genomic programming in human Langerhans cells we performed whole transcriptome (bulk RNA-seq and single cell RNA-seq) profiling and analysis of H3K4Me3 and H3K27Ac histone modifications across LC genome in primary human cells from 6 independent donors. Primary LCs were either unstimulated and stimulated with TNF-alpha. Additionally we performed a CRISPR editing experiment for IRF4 SOURCE: Marta,Ewa,Polak (m.e.polak@soton.ac.uk) - Systems Immunology Group University of Southampton