We sequenced mRNA from cardiomyocytes derived from hESCS in vitro.  By using the Cas9n, we generated the QKI null hESCs. The gene expression level and alternative splicing events were compared between 4 control and 4 QkI KO samples.  Here, we applied a widely used cardiomyocyte differentiation protocol  that was reported to produce a population of more than 90% cardiac troponin T (TNNT2)-positive cardiomyocytes. And we are able to demonstrate that QKI is indespensible to cardiac sarcomerogenesis and cardiac function through its regulation of alternative splicing in genes involved in Z-disc formation, such as ACTN2, NEBL, ABLIM1, and PDLIM5. SOURCE: Weinian Shou (wshou@iu.edu) - Shou Indiana University

Pluto Bioinformatics

GSE144008: Genome wide gene-expression and mRNA splicing profile in human cardiomyocytes differentiated from Wt and QKI mutant embryonic stem cells