Pluto Bioinformatics

GSE154996: Conservedinterferon-gamma signaling and decreased immune exclusion programs in responses to immune checkpoint blockade therapy

Bulk RNA sequencing

Purpose: We treated melanoma cell lines with IFNG to assess which ones would be resistant or responsive to T-cell attack.; Methods: We treated the melanoma cell lines with IFNG and then sequenced them at 6 hours to assess changes the transcriptome.; Results: Unless the cell lines had JAK1 or JAK2 KO, they responded very consistently with human melanocytes (normals), revealing a consistent signature for IFNG response in melanoma.; Conclusions: IFNG signaling is conserved in melanoma cell lines, and there are genes that go down in IFNG that allow immune infiltration to make immune blockade response possible. SOURCE: Antoni Ribas (aribas@mednet.ucla.edu) - University of California, Los Angeles