Differentiated somatic cells can be reprogrammed into induced pluripotent stem cells by ectopic expression of transcription factors Oct4, Sox2, Klf4, and c-Myc, but the mechanisms are still to be dissected. The stoichiometry of factors influences the efficiency of induced pluripotent stem cells, and previous studies emphasized the requirement of high levels of overexpressed Oct4. In this study, we showed that, with appropriate stoichiometry achieved by polycistronic cassettes, Sox2 and Klf4 were sufficient to initiate and establish pluripotency in differentiated cells efficiently without Oct4 overexpression. SOURCE: Sheng Ding (sheng.ding@gladstone.ucsf.edu) - Ding lab Gladstone Institute for Cardiovascular Disease

Pluto Bioinformatics

GSE98280: Function of Sox2 and Klf4 during SKM reprogramming