Pluto Bioinformatics

GSE130143: FIN-Seq: Transcriptional profiling of specific cell types in frozen archived tissue from the human central nervous system

Bulk RNA sequencing

FIN-Seq is a method that allows transcriptional profiling of specific cell types in frozen postmortem human CNS tissues. Briefly, nuclei from frozen CNS samples are isolated, fixed, and immunolabeled with antibodies for prospective isolation. The RNA from the FACS isolated nuclei are extracted and an RNA sequencing library is generated for subsequent sequencing. SOURCE: Ryoji Amamoto (ryoji_amamoto@hms.harvard.edu) - Cepko Harvard Medical School