Pluto Bioinformatics

GSE128743: EBF-1 mutant bone marrow stroma confers long-term changes in hematopoietic stem cell potential

Bulk RNA sequencing

Here, we investigate the role of Early B-cell factor 1 (EBF1) in MSCs to support hematopoiesis. Ebf1 deletion in Cxcl12-abundant reticular (CAR) cells and PDGFR+Sca1+CD45-CD31-Lin- (PS) cells in the bone marrow decreases the numbers of HSCs and myeloid cells. Single cell and bulk transcriptome analysis, combined with analysis of chromatin accessibility in EBF1-deficient MSCs revealed decreased expression of adhesion molecules and altered niche composition. In consequence, HSCs exposed to the EBF1-deficient niche, exhibit altered chromatin accessibility and impaired occupancy by myeloid regulators like AP-1, ETS and IRF. SOURCE: Josip,Stefan,Herman (herman@ie-freiburg.mpg.de) - Research Group Dominic Grün Max Planck Institute of Immunobiology and Epigenetics