Pluto Bioinformatics

GSE94963: Human Adult Sorted Live Cell Erythroblasts transduced with Sigma shRNA Clone TRCN0000162889 targeting SLC25A39 with puromycin selection RNAseq.

Bulk RNA sequencing

Erythroblasts cultured from mobilized CD34+ cells from six healthy adult human donors were used to generate an erythroblast transcriptome transduced with Sigma shRNA Clone TRCN0000162889 targeting SLC25A39 gene. Cellular maturation was maintained including enucleation. On culture day 14 total RNA was isolated (see PMID: 23798711 for details). These RNA-Seq profiles were generated after flow cytometric sorting (live cell gating of culture Day 14 erythroblasts according to forward and side scatter). Preliminary screening data demonstrated a robust increase in fetal hemoglobin that may be due to off-target effects from this shRNA clone. See Series GSE94147 for Empty Vector Control dataset. SOURCE: Jeffery,L.,Miller (jm7f@nih.gov) - Molecular Genomics and Therapeutics Section, Genetics of Development and Disease Branch NIH