Pluto Bioinformatics

GSE60630: RNA-Seq to assess the transcriptional effects of G quadruplex stabilization by the G4 ligand PhenDC3 in HT-1080 cells

Bulk RNA sequencing

G quadruplex motifs are frequently located near TSS and in the first introns of transcribed genes. We investigated the role that G4 structures play in transcription by stabilizing G4 DNA with the selective G4 ligand PhenDC3 in the HT-1080 human fibrosarcoma cell line. After treatment of triplicate samples with PhenDC3 or a negative control (DMSO carrier only), we performed RNA-Seq after poly-dT selection to assess changes in gene and isoform expression, as well as changes to splicing patterns. SOURCE: Lucas,T,Gray (lucasg@alleninstitute.org) - Tasic Allen Institute for Brain Science