Pluto Bioinformatics

GSE98027: Changes in gene expression due to alpha-crystallin mutations cryaa-R49C and cryab-R120G in mutant knock-in mouse lenses

Bulk RNA sequencing

To investigate the relationship between histones, chaperone function, and cataracts, we performed RNA-seq, isothermal titration calorimetry (ITC), size-exclusion chromatography, and gel electrophoresis of histones. The RNA-seq of postnatal lenses from 2-day-old cryaa-R49C mice revealed increased histone gene expression, suggesting that a -crystallin mutation regulates histones via a transcriptional mechanism. SOURCE: Usha Andley (andley@vision.wustl.edu) - Andley Washington University School of Medicine