Pluto Bioinformatics

GSE85161: DHX9 suppresses spurious RNA processing defects originating from the Alu invasion of the human genome [RNA-Seq]

Bulk RNA sequencing

Transposable elements increase genetic diversity thus making them an important part of evolution and gene regulation in all organisms that carry these sequences. Bulk of our nascent transcriptome is comprised of transposable elements that have the propensity to form strong secondary structures. It is essential to resolve such strong secondary structures to maintain normal cellular function. Here, we show that the major nuclear RNA helicase DHX9/RHA interacts and remodels embedded Alu retrotransposable elements in the human transcriptome and B1 retrotransposable elements in the mouse transcriptome.; To understand the effect of loss of DHX9 in human transcriptome we performed knockdown of DHX9 in HEK293 cells using two different siRNAs, followed by polyA-plus and polyA minus RNA extraction and sequencing. SOURCE: Asifa Akhtar (akhtarlab_data@ie-freiburg.mpg.de) - Akhtar Lab Max Planck Institute of Immunobiology and Epigenetics