Pluto Bioinformatics

GSE106928: A lysosome-plasma membrane-sphingolipid axis linking lysosomal storage to cell growth arrest

Bulk RNA sequencing

We used human fibroblasts loaded with sucrose as a simple model of lysosomal accumulation, and we verified by high-throughput RNA sequencing which processes were altered by the treatment at two different time points. Moreover, we hypothesized that ceramide production due to the ectopic catabolism of plasma-membrane glycosphingolipids is involved in cell cycle arrest upon sucrose treatment. To test this hypothesis, we treated sucrose-loaded fibroblasts with two specific inhibitors for the -glucosidases GBA1 and GBA2, and analyzed the cell responses using high-throughput RNA sequence. SOURCE: Giulia Solda' (giulia.solda@hunimed.eu) - Humanitas University