Pluto Bioinformatics

GSE84068: Post-transcriptional 3UTR cleavage of mRNA transcripts generates thousands of stable uncapped autonomous RNA fragments

Bulk RNA sequencing

Here, using genome wide analysis, we demonstrate that canonical mRNA is processed post-transcriptionally through an alternative cleavage and polyadenylation mechanism. As a result of this process, the downstream cleavage fragment of the 3UTR remains uncapped and stable; This finding indicates that different parts of gene mRNA are separate and independent, by re-annotating the human transcriptome using this model, we provide a new overview of the function and impact of microRNA (miRNA); Our results shed new light on the mammalian transcriptome and show that what were considered as 3UTRs are in fact autonomous RNA fragments. SOURCE: Tommy Kaplan (tommy@cs.huji.ac.il) - Hebrew University