Pluto Bioinformatics

GSE131512: Extracellular RNA in a single droplet of human serum reflects physiologic and disease states

Bulk RNA sequencing

We developed SILVER-seq (Small Input Liquid Volume Extracellular RNA Sequencing) to efficiently sequence both integral and fragmented cfRNAs from a small droplet (5-7 microliters) of liquid biopsy. We carried out SILVER-seq on more than 150 serum droplets from male and female donors ranging from 19 to 48 years of age. SILVER-seq detected cfRNAs from more than a quarter of the human genes, including small RNAs and fragments of mRNAs and long non-coding RNAs (lincRNAs). SOURCE: Sheng Zhong UCSD