Purpose: The goals of this study are to verify the inhibition of Wnt signaling is suffice to surpress the expression of MAGs in H1 cells during human early hematopoietic differentiation through comparing the transcriptome profilings in WT samples and samples with IWP2 treatment collected at day 2 after hematopoietic differentiation.;	Methods: mRNA profiles of hESC samples collected at day 2 after hematopoiesis differentiation were generated by deep sequencing using Illumina GAIIx. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: BurrowsWheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRTPCR validation was performed using TaqMan and SYBR Green assays;	Conclusions: Inhibiting Wnt signaling is suffice to significantly surpressed the expression of MAGs. SOURCE: Jiaxi Zhou (leisheng_zhang@163.com) -  PUMC

GSE134910: RNA Sequencing Facilitates Quantitative Analysis of Transcriptomes of H1 derived cells with or without IWP2 treatment at Day2 after human early hematopoietic differentiation .

Pluto Bioinformatics

GSE134910: RNA Sequencing Facilitates Quantitative Analysis of Transcriptomes of H1 derived cells with or without IWP2 treatment at Day2 after human early hematopoietic differentiation .