Pluto Bioinformatics

GSE147423: Transcriptomic analysis of day 1 (D1) differentiating WT and HDAC11-deficient mouse myoblasts

Bulk RNA sequencing

To get insight into the transcriptomic changes caused by HDAC11 loss at early myogenic differentiation, we performed RNA sequencing (RNA-Seq) in 24 h differentiated primary myoblasts from WT and HDAC11-deficient mice. Satellite cell-derived primary myoblasts were obtained after FACS isolation of skeletal muscle cells by growing them at low confluence in proliferation medium. To induce myoblast differentiation, cells were plated at high confluence and changed to differentiation media. Gene Ontology (GO) analysis of the genes upregulated in HDAC11 KO differentiating myoblasts revealed a statistically significant enrichment of cell cycle-related processes while the downregulated genes in HDAC11 KO differentiating myoblasts were enriched in muscle system process and muscle contraction GO categories. SOURCE: Lauro Sumoy (lsumoy@igtp.cat) - IGTP