Lef1 is a critical transducer of the Wnt/beta-catenin signaling pathway that is essential for mesoderm differentiation in vertebrate embryos. We established a doxycycline inducible ES cell line to over-express Flag-tagged Lef1 in differentiating ES cells which have the capacity to form mesoderm cells in vitro. The goal of this study was to identify the genes/gene networks regulated by Lef1 to understand how it regulates mesoderm differentiation and to establish the Lef1-regulated, Wnt/beta-catenin transcriptome. SOURCE: Mark,W,Kennedy (mark.kennedy@nih.gov) - Cell Signaling in Vertebrate Development NIH/NCI

Pluto Bioinformatics

GSE73083: Determination of the Lef1 regulated tanscriptome in differentiating ES cells