Pluto Bioinformatics

GSE147376: RNA-seq analysis of pulmonary arterial smooth muscle cells(PASMCs)stimulated with BMP9/10

Bulk RNA sequencing

Inactivation of BMP9/10 in mice leads to attenuated contractility of smooth muscle cells and decreased blood pressure.Treatment of de-differentiated PASMCs with BMP9/10 resulted in a strong increase of ACTA2 expression as measured by immunofluorescence and RT-PCR, indicating a switch from the de-differentiated, synthetic to a differentiated, contractile state. To further study the role of BMP9/10 in smooth muscle cells, we isolate PSAMCs and stimulate cells with or without BMP9/10 and perform the RNA-seq analysis. SOURCE: Mario Looso (mario.looso@mpi-bn.mpg.de) - Max Planck Institute for Heart and Lung Research