Pluto Bioinformatics

GSE123187: Single-cell and spatial transcriptomics reveal somitogenesis in gastruloids

Bulk RNA sequencing

Gastruloids are three-dimensional aggregates of embryonic stem cells (ESCs) that display key features of mammalian post-implantation development, including germ layer specification and axial organization. Gastruloids have mostly been characterized with microscopy-based approaches, limiting the number of genes that can be explored. It is therefore unclear to what extent gene expression in gastruloids reflects in vivo embryonic expression. Using both single-cell RNA-seq (scRNA-seq) and spatial transcriptomics we systematically compared cell types and spatial expression patterns between mouse gastruloids and mouse embryos. SOURCE: Anna Alemany (a.alemany@hubrecht.eu) - AVO Hubrecht Institue