The effort to better understand intestinal stem cell (ISC) identity and regulation remains a challenge.  We have been studying the RNA-binding protein MEX3A as a putative ISC marker. In that context, we have generated the first Mex3a knockout (KO) mouse model and show MEX3A is crucial for maintenance of the Lgr5+ ISC pool. As part of a phenotypic characterization pipeline, we have performed transcriptomic profiling (RNA-sequencing) of isolated Mex3a KO small intestinal crypts and compared it against small intestinal crypts isolated from age-matched wild-type controls. SOURCE: Bruno Pereira (bpereira@ipatimup.pt) - Differentiation and Cancer i3S/IPATIMUP

Pluto Bioinformatics

GSE141191: Transcriptomic profiling of small intestinal crypts upon MEX3A deletion