Pluto Bioinformatics

GSE143371: Transcriptome analysis of differentiated human embryonic stem cells (hESCs) upon CRISPR/Cas9-mediated mutagenesis of lineage-specific and common essential genes

Bulk RNA sequencing

Pluripotentstemcells can differentiate into all embryonic germ layers, a process that is orchestrated by the dissolution of pluripotency network and activation of pro-differentiation pathways. We established a genome-wide loss-of-function library in haploid hPSCs and differentiated these cells into the three germ layers. The analyses of our high-throughput genetic screening revealed germ layer-specific essential transcription factors, as well as common essential genes for the transition from pluripotency into the three embryonic germ layer fates. Here, we have generated individual knockouts of seven lineage-specific and four common essential genes in human embryonic stem cells by CRISPR/Cas9 mutagenesis and analyzed the transcriptomes of the mutants upon either directed or spontaneous differentiation. SOURCE: Carmel Braverman-Gross (carmel.braverman@mail.huji.ac.il) - Nissim Benvenisty Hebrew University of Jerusalem