Pluto Bioinformatics

GSE72862: Next Generation Sequencing Facilitates Quantitative Analysis of mesoderm posterior bHLH transcription factor 1(MESP1)+ and MESP1- cells' Transcriptomes

Bulk RNA sequencing

Purpose: To compare the transcriptome of MESP1-mTomato reporter cells at undifferentiated state, cardiac differentiation day 3 and day 5.; ; Methods: total RNA from sorted MESP1+, MESP1- and HESCs (in biological duplicates) was extracted using RNeasy Plus Mini Kit (Qiagen) and treated with RNase free DNase. RNA library was prepared following the instruction of TruSeq RNA Sample Preparation kit (Illumina) and sequenced on Illumina HiSeq 2000.; ; Results: genes differentially expressed in MESP1-mTomato+ and mTomato- cells were identified.; ; Conclusions: the gene expression profile of MESP1-mTomato cells indicates that they are cardiovascular progenitor cells. SOURCE: Jie Na (jie.na@tsinghua.edu.cn) - Jie Na Tsinghua University