Pluto Bioinformatics

GSE58757: Extensive crosstalk between lncRNAs and mRNAs in mouse stem cells

Bulk RNA sequencing

To determine the temporal variation of mRNA levels, we collected and sequenced poly-adenylated RNA from all cell extracts, cytoplasmic and nuclear fractions of a conditional Dicer mutant [DTCM23/49 XY (Nesterova et al. 2008)] mouse Embryonic Stem Cells before induction of Dicer excision (day 0) and at days 4, 8, 10 and 12 following Dicer loss of function. coverage. SOURCE: Ana Marques (ana.marques@dpag.ox.ac.uk) - University of Oxford