RNA sequencing analysis of control and ERK3 knockdown  HCPECs was performed to understand the physiological relevance of ERK3 in maintaining basal as well as LPS-mediated innate immune responses in HCPECs. Results revealed that ERK3 is required for the regulation of several key cytokines and chemokines even at the steady state.;	In particular, we discovered that ERK3 is indespansable for the transcriptional regulation of CXCL8/IL-8, CXCL10, MCP-1/CCL2 and GCP-2/CXCL6. Concomitantly, a functional interpretation of the differentially expressed (DE) genes with topGO indicated an important role of ERK3 in regulation of genes involved in, among others, immune responses and leukocyte chemotaxis. SOURCE: Federico Marini (marinif@uni-mainz.de) -  University Medical Center - Mainz

Pluto Bioinformatics

GSE136002: Transcriptome analysis of control and ERK3-depleted Human Colonic Primary Epithelial cells (HCPECs)