Traditional single-cell RNA-seq technologies have shown limitations for large scale experiments. Here, we constructed a single-cell resolution transcriptomic atlas of (HSPCs) in human and mouse,including a total number of 71,729 single cells clustered into 39 subpopulations by computational analysis. In human, we found 20 progenitors primed with unique lineages like megakaryocyte, neutrophil and B cell progenitor can project directly from HSPCs. Within the c-Kit positive cells from mouse bone marrow, we described 19 progenitor subgroups at single cell resolution. SOURCE: guoji guo (ggjlab@163.com) - guoji guo lab Zhejiang University School of Medicine

Pluto Bioinformatics

GSE92274 (human): Comparative Transcriptomic Analysis of Hematopoietic System Across Species by Microwell-Seq