Pluto Bioinformatics

GSE134818: Comparative analysis of mRNA distribution in heavy and light polysomes in wild type and Dicer1 knockout cells

Bulk RNA sequencing

To gain a global view of translational inhibition by microRNA (miRNA), we isolated polysomes from wild type and Dicer1 knockout HCT116 human cells using sucrose gradient fractionation technique. The polysome fraction was separated into light (9mer or less) and heavy (10mer or more) subfractions. RNA samples were extracted from both subfractions and subjected to RNA-seq analysis. A general shift from light to heavy subfractions was detected for miRNA targted mRNAs. SOURCE: Degeng Wang Wang (degeng.wang@ttu.edu) - Texas Tech University