In vivo, melanoma cells transition though distinct phenotypic states in response to a changing microenvironment, and most notably can switch between invasive and proliferative phenotypes characterized by high and low levels of MITF activity (Hoek and Goding, 2010; Hoek et al., 2008). Since melanoma cell lines isolated from human tumors tend also to fall into either proliferative or invasive, slow-growing phenotypes (Hoek et al., 2006), it seems likely that established lines reflect specific phenotypic states within tumors, including those detected using single cell RNA-seq, that are then fixed and maintained under nutrientrich culture conditions where the microenvironmental stresses encountered in vivo are absent. Indeed, it has been shown that melanoma cell lines presenting distinct phenotypic states exhibit very different responses to microenvironmental cues such as hypoxia (Louphrasitthiphol et al., 2019). The aim of this study is to characterise some of the commonly use cell lines and assign each with a phenotypic state base on its transcriptomics. SOURCE: Pakavarin Louphrasitthiphol (pakavarin.louphrasitthiphol@ludwig.ox.ac.uk) - Prof. CRGoding Ludwig Institute for Cancer Research

Pluto Bioinformatics

GSE137391: Transcriptomics profiling of some commonly used cell lines at the base-line culture condition