Pluto Bioinformatics

GSE119596: High-throughput RNA-sequencing-based transcriptomic profiles of embryonic lens development for cataract gene discovery

Bulk RNA sequencing

We applied previously established in silico whole-embryo body (WB)-subtraction-based approach to identify lens-enriched genes. These new RNA-seq datasets on embryonic stages E10.5, E12.5, E14.5 and E16.5 confirmed high expression of established cataract-linked genes and identified several new potential regulators in the lens. Finally, we present lens stage-specific UCSC Genome Brower annotation-tracks; these are publicly accessible through iSyTE (https://research.bioinformatics.udel.edu/iSyTE/) and enable a user-friendly visualization of lens gene expression/enrichment to help prioritize genes from high-throughput data from cataract cases. SOURCE: Salil,A,Lachke (anand@udel.edu) - University of Delaware