The depletion of HMGB1 reduces the deposition of histones on naked DNA (Celona et al, 2011). in primary MEFs and yeast. We verified that the downmodulation of HMGB1 decreases histone level also in a model of murine mesothelioma.  HMGB1 was KD in Murine Mesothelioma AB22 cells by the expression of a shRNA. Nucleosome positioning and occupancy were tested by ATAC-seq. Since a reduced amount of nucleosomes increase the cell sensitivity to gamma irradiatio, we tested the number and position of breaks using BLISS-seq. SOURCE: Jose,Manuel,Garcia-Manteiga (garciamanteiga.josemanuel@hsr.it) -  OSR - San Raffaele Scientific Institute

Pluto Bioinformatics

GSE134795: Nucleosomes protect DNA from irradiation-induced double strand breaks in living cells (RNA-seq)