Cell fixation, permeabilization and antibody staining of could have adverse effects on the quality of single cell transcriptomics data. To assess the effects of the RAID procedure, which includes such steps, we performed a direct comparison of single cell transcriptomics by CELseq2 using unfixed and RAID-processed cells.  Quality measures (gene complexity, gene detection rate, average gene expression) were performed using 40000 samples UMI counts per cell. SOURCE: Jan,Patrick,Gerlach (janpgerlach@gmail.com) -  Radboud Institute for Molecular Life Sciences

GSE115861: Comparison of single-cell transcriptomics quality between unfixed cells and cells that were fixed and mock stained according to the RAID procedure

Pluto Bioinformatics

GSE115861: Comparison of single-cell transcriptomics quality between unfixed cells and cells that were fixed and mock stained according to the RAID procedure